Determination of the molecular mass of new L-fucose-containing glycosaminoglycan and its distribution by high-performance gel-permeation chromatography with laser light-scattering detection.
نویسندگان
چکیده
منابع مشابه
Fractionation and characterization of phenolic resins by high-performance liquid chromatography and gel-permeation chromatography combined with ultraviolet, refractive index, mass spectrometry and light-scattering detection.
HPLC and gel permeation chromatographic (GPC) characterization of complex phenol-formaldehyde resins is described. Reversed-phase HPLC fingerprints the phenolic monomers, dimers and some oligomers. The molecular masses of these phenolic compounds were determined using an ion trap mass spectrometer. GPC analyzes tetrahydrofuran-soluble phenolic polymers beyond HPLC capability. The molecular mass...
متن کاملUse of low-angle laser light scattering with gel permeation chromatography for the molecular mass determination of biomolecules.
486 Use of low-angle laser light scattering with gel permeation chromatography for the molecular mass determination of biomolecules K. Jumel,* P. Br0wne.t and J. F. Kennedy# *Chembiotech Ltd, Institute of Research and Development, Vincent Drive, Edgbaston, Birmingham B I5 2sQ, U.K.; tLDC Analytical, Diamond Way, Stone Business Park, Stone, Staffs STI 5 OHH, U.K. and $Research Laboratory for the...
متن کاملDetection of serum proteins by high-pressure gel-permeation chromatography with low-angle laser light scattering, compared with analytical ultracentrifugation.
Human sera were subjected to analytical ultracentrifugation and "high-pressure" gel-permeation chromatography on a system of combined TSK Gel G5000 PW and G3000 SW columns. The chromatographic method produced remarkably superior resolution of the proteins, especially those exceeding 100 000 Da. We calculated the molecular masses of eluted fractions on the basis of their detection by low-angle l...
متن کاملIdentification of Colored Components Produced in Sugar Beet Processing Using Gel-Permeation Chromatography (GPC) with UV and RI Detection
Sugar juices in the production of beet sugar contain complex varieties of colored materials that have not been identified completely. Color of the sugar depends the color of juices from which sugar is produced. The mechanisms concerning color formation in beet sugar processing are complicated due to many parameters involved. The main mechanisms related to the color formation during purification...
متن کاملDetermination of the absolute molecular weight averages and molecular weight distributions of alginates used as ice cream stabilizers by using multiangle laser light scattering measurements.
High-performance size exclusion chromatography with multiangle laser light scattering detection (HPSEC-MALLS) was used for characterizing complete molecular weight distributions for a range of commercial alginates used as ice cream stabilizers. For the samples investigated, molecular weight averages were found to vary between 115 000 and 321 700 g/mol and polydispersity indexes varied from 1. 5...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
دوره 17 4 شماره
صفحات -
تاریخ انتشار 2001